Advanced OAT Test India — 140+ Metabolic Biomarkers
in One Urine Test
The Advanced Organic Acids Test (OAT) uses LC-MS/MS technology to evaluate 140+ urinary metabolites across 9 critical functional pathways — gut microbiome, mitochondrial energy, neurotransmitters, nutritional status, amino acids, detoxification, oxidative stress, methylation, and fatty acid metabolism. One urine sample reveals the root cause of chronic, unexplained conditions.
What Is the Advanced OAT Test?
The Advanced Organic Acids Test (OAT) is a comprehensive urine-based metabolomics profile that measures over 140 organic acid biomarkers and calculates 21 functional ratio indices using Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS) — the gold standard in metabolomics analysis. Unlike conventional blood panels that measure static nutrient levels, the Advanced OAT Test evaluates the functional activity of metabolic pathways — revealing whether critical cellular processes are actually working optimally, regardless of what blood values suggest.
Organic acids are small molecules produced as byproducts of normal metabolism in the body's cells, gut microbes, and detoxification pathways. When these metabolites accumulate abnormally in urine, they signal disruptions in the underlying pathways — even before clinical symptoms become severe enough to show on standard tests. The Advanced OAT Test captures this early, functional picture, making it one of the most clinically valuable investigations available in functional and integrative medicine today.
What the Advanced OAT Test Evaluates — 9 Clinical Domains
The Advanced OAT Test provides a systems biology view of your metabolism — evaluating nine interconnected functional domains in a single urine sample:
Gut Microbiome & Dysbiosis
Measures yeast, fungal, bacterial, and Clostridia metabolites — identifying overgrowths that drive inflammation, leaky gut, and systemic symptoms far removed from the gut itself.
Mitochondrial Function & Energy
Evaluates Krebs cycle intermediates and oxidative phosphorylation markers — identifying cellular energy deficits that underlie chronic fatigue, brain fog, and poor exercise tolerance.
Neurotransmitter Metabolism
Assesses dopamine, serotonin, and kynurenine pathway metabolites — revealing imbalances linked to anxiety, depression, ADHD, autism, and mood dysregulation.
Nutritional & Vitamin Function
Detects functional deficiencies of B6, B12, folate, B5, CoQ10, and vitamin C — even when standard serum levels appear normal — by measuring their metabolic downstream markers.
Amino Acid Metabolism
Profiles essential and non-essential amino acids plus branched-chain ketoacid catabolism markers — identifying protein synthesis deficits, metabolic errors, and enzyme deficiencies.
Detoxification & Oxidative Stress
Measures glutathione status, lipid peroxidation, DNA oxidative damage (8-OHdG), environmental toxin exposure (BPA, xylene, acrolein), and nitrogen metabolism — assessing total toxic burden.
Fatty Acid & Ketone Metabolism
Evaluates beta-oxidation efficiency, ketone body production, and fatty acid transport — identifying metabolic blocks that impair energy production from fats.
Methylation & One-Carbon Metabolism
Measures folate metabolism (FIGLU), methionine cycle activity, pyrimidine metabolites, and methylation cofactor status — critical for gene expression, DNA repair, and neurological health.
Functional Ratio Indices (21)
Calculates 21 inter-pathway ratios — including mitochondrial efficiency ratios, neurotransmitter balance indices, and redox ratios — that provide clinical insight beyond individual metabolite values.
Complete Advanced OAT Test Marker List — 140+ Biomarkers
Every biomarker measured by the Advanced OAT Test, organised by functional category:
🦠 Yeast & Fungal Markers [9]
- Citramalic acid
- 5-Hydroxymethyl-2-Furoic acid (Aspergillus)
- 3-Oxoglutaric acid
- Furan-2,5-dicarboxylic acid (Aspergillus)
- Furancarbonylglycine (Aspergillus)
- Tartaric acid (Aspergillus)
- Arabinose
- Carboxycitric acid
- Tricarballylic acid (Fusarium)
🧫 Bacterial Markers [9]
- Hippuric acid
- Phenylacetic acid
- 2-Hydroxyphenylacetic acid
- 4-Hydroxybenzoic acid
- Benzoic acid
- Benzoylformic acid
- DHPPA
- Trimethylamine-N-Oxide (TMAO)
- 4-Hydroxybenzoic acid (isomer)
🔴 Clostridia Markers [5]
- 4-Hydroxyphenylacetic acid (C. difficile)
- HPHPA (C. sporogenes, C. botulinum)
- p-Cresyl sulfate (C. difficile)
- 3-Indoleacetic acid (Clostridia spp.)
- 3-Hydroxypropionic acid
🟡 Oxalate Metabolites [3]
- Glyceric acid
- Glycolic acid
- Oxalic acid
⚡ Krebs Cycle Metabolites [7]
- Succinic acid
- Fumaric acid
- Malic acid
- α-Ketoglutaric acid
- Cis-aconitic acid
- Citric acid
- Isocitric acid
🔋 Glycolytic & Mitochondrial [9]
- Lactic acid
- Pyruvic acid
- 3-Methylglutaric acid
- 3-Methylglutaconic acid
- 2-Hydroxyglutaric acid
- 2-Methylglutaric acid
- 3-Hydroxyglutaric acid
- Succinylacetone
- NAD+ (Nicotinamide adenine dinucleotide)
🧠 Neurotransmitter Metabolites [9]
- Vanilmandelic acid (VMA — norepinephrine/epinephrine)
- Homovanillic acid (HVA — dopamine)
- HVA / VMA Ratio
- Dihydroxyphenylacetic acid (DOPAC)
- HVA / DOPAC Ratio
- 5-HIAA (serotonin)
- 2,3-Pyridinedicarboxylic acid
- Kynurenic acid
- Picolinic acid
💊 Nutritional Markers [10]
- Methylmalonic acid (Vitamin B12)
- FIGLU (Folate marker)
- Pyridoxic acid (Vitamin B6)
- Pantothenic acid (Vitamin B5)
- Glutaric acid
- Ascorbic acid (Vitamin C)
- 3-HMG (CoQ10 marker)
- Mevalonic acid (Cholesterol biosynthesis)
- N-Acetylcysteine (Glutathione precursor)
- 2-Methylcitric acid (B12/propionyl-CoA)
🛡️ Detoxification Markers [18]
- Pyroglutamic acid (Glutathione/Redox)
- Glutathione Reduced (GSH)
- Glutathione Disulfide (GSSG)
- GSH/GSSG Ratio
- 2-Hydroxybutyric acid
- Orotic acid (Ammonia excess)
- 2-Hydroxyhippuric acid
- 2-Methylhippuric acid (Xylene)
- 3-/4-Methylhippuric acid (Xylene)
- 8-OHdG (DNA oxidative damage)
- 8-Hydroxyguanine
- Bisphenol A (BPA)
- Bisphenol B (BPB)
- Acrolein
- 3-Nitrotyrosine
- Malondialdehyde (Lipid peroxidation)
- 1,4-Dihydroxynonane mercapturic acid
- 8-iso-Prostaglandin F2α
🔥 Fatty Acid & Ketone Markers [9]
- 3-Hydroxybutyric acid
- Acetoacetic acid
- Adipic acid
- Suberic acid
- Sebacic acid
- Ethylmalonic + Methylsuccinic acid
- Malonic acid
- Tiglylglycine
- 2-Pimelic acid
🧬 Amino Acids — Essential [8]
- Histidine
- Isoleucine
- Leucine
- Lysine
- Methionine
- Phenylalanine
- Threonine
- Valine
🧬 Amino Acids — Non-Essential [9]
- Alanine
- Arginine
- Aspartic acid
- Cystine
- Glutamic acid
- Glycine
- Proline
- Tyrosine
- Serine
⚗️ Branched-Chain & Catabolism [20]
- 2-Hydroxyisovaleric acid
- α-Ketoisovaleric acid
- 3-Methyl-2-oxovaleric acid
- 2-Hydroxyisocaproic acid
- α-Ketoisocaproic acid
- 2-Oxo-4-methiolbutyric acid
- Mandelic acid
- 3-Phenyllactic acid
- Phenylpyruvic acid
- Homogentisic acid
- 4-Hydroxyphenyllactic acid
- 4-Hydroxyphenylpyruvic acid
- Xanthurenic acid
- 3-Hydroxyisovaleric acid
- N-Acetyl-L-aspartic acid
- α-Ketobutyric acid
- 4-Hydroxybutyric acid
- O-Phosphorylethanolamine
- Creatine
- Argininosuccinic acid
🔗 Acylglycines [10]
- Butyrylglycine
- Isobutyrylglycine
- 3-Methylcrotonylglycine
- 2-Methylbutyrylglycine
- Isovalerylglycine
- Valerylglycine
- Hexanoylglycine
- 3-Phenylpropionylglycine
- Suberylglycine
- Other acylglycines
21 Functional Pathway Ratio Indices — Unique to Advanced OAT
Beyond raw metabolite values, the Advanced OAT Test calculates 21 clinically derived ratio indices that reveal the balance and efficiency of interconnected biochemical systems — providing insights that individual measurements alone cannot:
- Lactate / Pyruvate
- Citrate / cis-Aconitate
- cis-Aconitate / Isocitrate
- α-KG / Succinate
- Succinate / Fumarate
- Fumarate / Malate
- HVA / VMA
- HVA / DOPAC
- Kynurenic / Xanthurenic
- Kynurenic / Picolinic
- Quinolinate / Kynurenate
- Pyridoxic / Serotonin
- Pyridoxic / Kynurenic
- Pyridoxic / Xanthurenic
- MMA / FIGlu
- Methionine / 2-Hydroxybutyric
- Ascorbate / Pyroglutamate
- Glycine / Pyroglutamate
- Serine / Pyroglutamate
- Pantothenic / Pyroglutamate
- Orotate / Arginine
Who Should Consider the Advanced OAT Test?
The Advanced OAT Test is particularly valuable for individuals and families dealing with conditions that conventional tests have failed to explain — or for clinicians seeking the metabolic root causes beneath surface-level symptoms:
How the Advanced OAT Test Works — Step by Step
Order Kit & Confirm
Book on WhatsApp or call +91 89680 10467. We confirm your order and dispatch the OAT collection kit to your address anywhere in India within 1–2 working days.
Receive & Prepare
Fast for 10–12 hours (water permitted). Collect your first morning urine sample following the detailed instructions in the kit. No lab visit required.
Return Kit to Lab
Seal the sample as instructed and use the prepaid return shipping label provided. The dry-format sample is stable for transport — no cold chain required.
Report in 7 Working Days
Your comprehensive report — covering all 140+ biomarkers and 21 functional indices — is delivered digitally via email and WhatsApp with interpretation guidelines.
Advanced OAT Test vs Routine Blood Tests — What's Different?
| Feature | Routine Blood Tests | Advanced OAT Test |
|---|---|---|
| Sample type | Blood (venepuncture) | Urine — non-invasive |
| Biomarkers measured | 10–30 individual markers | 140+ metabolic markers |
| Technology | Standard biochemistry | LC-MS/MS (gold standard) |
| What it shows | Static nutrient/hormone levels | Functional pathway activity |
| Gut microbiome assessment | Not included | 23 gut microbial markers |
| Mitochondrial function | Not measured | 7 Krebs cycle + 9 additional |
| Neurotransmitter metabolism | Not included | 9 neurotransmitter metabolites |
| Toxin exposure markers | Not included | BPA, xylene, acrolein, 8-OHdG |
| Functional vitamin status | Serum levels only | Intracellular functional markers |
| Collection location | Lab visit required | Home — kit delivered to you |
| Personalised interventions | Generic supplementation | Targeted protocol development |
Advanced OAT Test for Autism — What It Reveals
The Advanced OAT Test has become a cornerstone investigation in functional medicine protocols for autism spectrum disorder (ASD). Research consistently confirms that children with autism show distinct metabolomic profiles — and the OAT Test identifies these with high specificity:
- Elevated Clostridia metabolites (HPHPA, 4-Hydroxyphenylacetic acid) — neurotoxic bacteria that block dopamine production, contributing to repetitive behaviours, irritability, and poor eye contact
- Elevated yeast markers (Arabinose, Tartaric acid) — Candida overgrowth depletes glutathione and impairs detoxification, worsening neurological symptoms
- Impaired mitochondrial function — low Krebs cycle intermediates indicate cellular energy deficits that drive fatigue, poor motor control, and cognitive difficulties
- Elevated oxidative stress (8-OHdG, Malondialdehyde) — oxidative damage to DNA and lipids, reflecting inadequate antioxidant defence
- Neurotransmitter imbalances — elevated HVA/VMA ratio, low serotonin metabolites, and kynurenine pathway dysregulation affecting mood and behaviour
- Glutathione depletion — impaired detoxification capacity measured directly through GSH/GSSG ratio
What Does an Advanced OAT Test Report Look Like?
The Advanced OAT Test report is a comprehensive clinical document — not a simple list of values. It is structured to guide both practitioners and informed patients through the results systematically:
- Individual metabolite values — each of the 140+ biomarkers reported with your measured value, reference range, and flagging of abnormal results
- 21 functional ratio indices — each ratio calculated and interpreted against established clinical ranges
- Pathway summary charts — visual heat maps showing which metabolic pathways are underperforming, overloaded, or balanced
- Clinical interpretation notes — explanatory text connecting biomarker patterns to likely clinical significance
- Intervention guidance — evidence-based nutritional, supplemental, and lifestyle recommendations aligned to your specific metabolic fingerprint
The report is delivered as a detailed PDF via email and WhatsApp within 7 working days of the lab receiving your sample. We strongly recommend reviewing it with a functional medicine practitioner who can contextualise results against your full clinical history.
Advanced OAT Test Preparation — What You Need to Know
Correct preparation is essential for an accurate Advanced OAT Test result. Certain foods, supplements, and medications can influence organic acid levels in urine — altering results and potentially leading to incorrect clinical conclusions. Follow these preparation guidelines:
- Antibiotics and antifungals — can suppress microbial markers
- High-dose B vitamin supplements (especially B6, B12)
- Probiotics — can artificially normalise gut markers
- Apple juice — contains malic acid which inflates OAT markers
- Grapes, raisins, cranberries — contain tartaric acid
- Alcohol — interferes with multiple pathways
- Fast for 10–12 hours (water only)
- Collect first morning urine upon waking
- Use only the collection materials in the kit
- Follow the exact instructions in the kit leaflet
- Store sample as directed until pickup or couriering
- Note any medications taken in the past 7 days
If you are unsure whether a specific supplement or medication should be paused before testing, contact our team at +91 89680 10467. We will provide specific guidance based on your situation.
Conditions the Advanced OAT Test May Help Evaluate
The Advanced OAT Test provides metabolic insight that is clinically relevant across a wide range of chronic and complex conditions. It is particularly valuable when conventional tests have returned normal results despite ongoing symptoms:
Advanced OAT Test — Frequently Asked Questions
Is the Advanced OAT Test a blood test?
What is the Advanced OAT Test price in India?
How long does fasting need to be before the OAT Test?
How is the kit delivered and how do I return the sample?
How many days does the Advanced OAT Test report take?
Can children take the Advanced OAT Test?
What technology does the Advanced OAT Test use?
Is the Advanced OAT Test ?
How is the Advanced OAT Test different from a standard Organic Acids Test?
Can I do the OAT Test while taking supplements?
Clinical Evidence & Why Practitioners Trust OAT Testing
Get the Complete Metabolic Picture — 140+ Biomarkers, Home Collection, Report in 7 Days
India's most comprehensive urine metabolomics test. Kit delivered to your home anywhere in India. Pay after booking confirmation. Results interpreted by functional medicine specialists.